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  • Abstract
  • Introduction
  • Lit. Review
  • Methods
    • Materials
  • Results
  • Conclusion
  • Acknowledgments
    • References
    • Contact
  • Home
  • Abstract
  • Introduction
  • Lit. Review
  • Methods
    • Materials
  • Results
  • Conclusion
  • Acknowledgments
    • References
    • Contact
The Isomerization of Humulone into Isohumulone

Results

H NMR was taken after the o-phenaline diamine complex was isolated to verify that we had obtained the desired complex, Figure 6.
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An H NMR was taken in deuterated chloroform after the isolation of humulone to verify that the complex was actually humulone, Figure 7.
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Another H NMR of humulone was taken of the humulone in deuterated acetone, Figure 8, instead of deuterated chloroform, Figure 7.  We did this to compare the impurities of the H NMR peaks and the location of the solvent peaks in relation to the humulone peaks.  
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A polarimeter reading was taken for the humulone that was dissolved in DI water with a pH of 10.  
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A polarimeter reading was taken of the humulone dissolved  in 8 mL of ethanol and 2 mL of pH 10 Ammonium Hydroxide Buffer.  
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An H NMR was also taken of the resulting compound after the pH 10 Ammonium Hydroxide Buffer and ethanol solution was added, Figure 9.  The resulting compound was believed to be a possible isohumulone.  
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An H NMR was taken of the isolated isohumulone, Figure 10.  Further purification of the compound is needed, but the differences between the isohumulone and possible isohumulone are still visible.  
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